Mouse VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

Mouse VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5
• UniProt: P55284
• Field of Research: CD & Adhesion molecule; Tumor immunity; Infection immunity.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 200 ng/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 3.13-200 ng/mL
• Sensitivity: 1.21 ng/mL