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Recombinant Human Granulocyte colony-stimulating factor protein (CSF3) (Active)

CAT: 0399-CSB-AP002071HU-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-AP002071HU-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
G-CSF, Filgrastim, Lenograstim
Abbreviation
Recombinant Human CSF3 protein (Active)
Gene Name
CSF3
UniProt
P09919
Expression Region
31-204aa
Organism
Homo sapiens (Human)
Target Sequence
TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP
Tag
Tag-Free
Type
Active Protein & In Stock Protein
Source
E.Coli
Field of Research
Immunology
Relevance
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
>98% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 107 IU/mg.
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 µm filtered 10mM sodium acetate buffer, containing 5 % trehalose, pH 4.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Molecular Weight
18.7 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein of Isoform Short

UniProtKB · P09919

Granulocyte colony-stimulating factor

CSF3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09919
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CSF3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
G-CSF
EC number
—
Processing
Precursor
Secondary accessions
A8MXR7
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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