EasyStep Human IGFBP1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit

EasyStep Human IGFBP1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP1, and the Human IGFBP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP1. After TMB substrate solution is added, only those wells that contain Human IGFBP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein
• Field of Research: Tumor immunity; Endocrinology; Cardiovascular biology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 1.5h
• Standard: 200 ng/mL
• Sample Type: Serum, plasma
• Detection Range: 3.13-200 ng/mL
• Sensitivity: 0.54 ng/mL