Eosin Y

CAT: 0246-AR-6522-04Size: 500 mLDry Ice: NoHazardous: No
CAT#:0246-AR-6522-04Size:500 mL
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Description
Hematoxylin and Eosin (H&E) staining is used most commonly in histology.
Applications
IHC
Assay Principle
H and E staining: Follow the procedure as outlined for H and E staining. We use the following protocol: 1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections. 2. Stain with hematoxylin for 4 minutes (2X), wash 4-5X with tap water, and rinse 2 X with de/di water. 3. Dip in 10 % acetic acid 2X, wash with de/di water, wash with Immuno Automation buffer (ImmunoBioScience AR-6561), PBS or any buffer with pH 7.4-8. 4. Rinse 2X with 70% ethanol, apply Eosin stain 1-5 minutes, and wash with 70% ethanol to remove excess Eosin.. 5. Rinse 2X with 95% ethanol, 2X with 100% ethanol, xylene or any dehydrating agent. 6. The slide can be mounted with organic mounting medium or our Organo /limonene (AR-6504) mounting medium.
Shipping Conditions
Ambient Temperature
Storage Conditions
Room Temperature

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