Recombinant Ophiophagus hannah Cathelicidin-related peptide Oh-Cath

Recombinant Ophiophagus hannah Cathelicidin-related peptide Oh-Cath

• CAS Number: 9000-83-3
• UniProt: B6S2X2
• Expression Region: 162-191aa
• Organism: Ophiophagus hannah (King cobra) (Naja hannah)
• Target Sequence: KFFKKLKNSVKKRAKKFFKKPRVIGVSIPF
• Tag: N-terminal 6xHis-SUMO-tagged
• Source: E.coli
• Field of Research: Others
• Assay Type: Developed Protein
• Relevance: Potent antimicrobial peptide against Gram-negative (MIC=0.25 ug/ml against E.coli ATCC 25922, MIC=0.5 ug/ml against P.aeruginosa) and Gram-positive bacteria (MIC=64 ug/ml against E.faecalis, MIC=64 ug/ml against S.aureus). Adopts an amphipathic alpha helical conformation, that may allow to partition into the target membrane. Low hemolytic activities have been observed on mammalian cells.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length of Mature Protein
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 21.8 kDa
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.