Recombinant Human ADGRE5 Protein (His Tag)

• Background:

  This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with an 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with an other EGF-TM7 genes on the short arm of chromosome 19.

• Abbreviation:

  ADGRE5

• Synonyms:

  ADGRE5; Adhesion G protein-coupled receptor E5; CD 97; CD_antigen=CD97; CD97; CD97 antigen; CD97 antigen subunit beta; CD97 molecule; CD97; Leukocyte antigen CD97; Seven span transmembrane protein; Seven transmembrane helix receptor; Seven transmembrane heterodimeric receptor associated with inflammation; TM7LN 1; TM7LN1

• UniProt:

  P48960

• Accession Number:

  P48960

• Expression System:

  Mammalian

• Tag:

  C-His

• Sequence:

  Gln21-Leu530

• Field of Research:

  Cancer; Immunology; Neuroscience; Signal Transduction; Stem Cells

• Endotoxin:

  < 1.0 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  55.9 kDa

• Observed Molecular Weight:

  80-100 kDa

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Human