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Recombinant Human SIN3-HDAC complex-associated factor (SINHCAF) (Active)

CAT: 0399-CSB-EP882059HUe0-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP882059HUe0-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Protein FAM60A (Tera protein homolog) (C12orf14) (FAM60A)
Abbreviation
Recombinant Human SINHCAF protein (Active)
Gene Name
SINHCAF
UniProt
Q9NP50
Expression Region
1-221aa
Organism
Homo sapiens (Human)
Target Sequence
MFGFHKPKMYRSIEGCCICRAKSSSSRFTDSKRYEKDFQSCFGLHETRSGDICNACVLLVKRWKKLPAGSKKNWNHVVDARAGPSLKTTLKPKKVKTLSGNRIKSNQISKLQKEFKRHNSDAHSTTSSASPAQSPCYSNQSDDGSDTEMASGSNRTPVFSFLDLTYWKRQKICCGIIYKGRFGEVLIDTHLFKPCCSNKKAAAEKPEEQGPEPLPISTQEW
Tag
N-terminal GST-tagged
Type
Active Protein & Developed Protein
Source
E.coli
Field of Research
Cancer
Relevance
Subunit of the Sin3 deacetylase complex, this subunit is important for the repression of genes encoding components of the TGF-beta signaling pathway . Core component of a SIN3A complex present in embryonic stem cells. Promotes the stability of SIN3A and its presence on chromatin and is essential for maintaining the potential of ES cells to proliferate rapidly, while ensuring a short G1-phase of the cell cycle, thereby preventing premature lineage priming.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized LCN2 at 2 μg/ml can bind human SINHCAF, the EC50 of human SINHCAF protein is 31.54-38.59 μg/ml.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
51.9 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length

UniProtKB · Q9NP50

SIN3-HDAC complex-associated factor

SHCAF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9NP50
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SINHCAF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
D3DUV8, Q9BSZ8
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome

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