Anti-Glutathione-S-Transferase (GST) Antibody

Anti-Glutathione-S-Transferase (GST) Antibody

• Description: Rabbit Polyclonal Antibody specific to Glutathione-S-transferase (GST)
• Product Name Alternative: GST 28
• CAS Number: 9007-83-4
• UniProt: P26624
• Host: Rabbit
• Reactivity: Glutathione-S-Transferase (Gst)
• Immunogen: Glutathione-S-Transferase (GST) from Schistosoma japonicum.
• Target Antigen: Glutathione S-transferase class-mu 28 kDa isozyme
• Target: Glutathione-S-Transferase (GST)
• Clonality: Polyclonal
• Isotype: Whole IgG
• Type: Antibody
• Applications: WB, IP, ICC, ELISA
• Field of Research: Epitope Tags
• Purification Method: Purified by affinity on GST
• Concentration: 1.0 mg/mL
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
• Additionnal Information: Immunoblotting: use at a dilution of 1:1,000- 1:20,000.
  
  Immunocytochemistry: use at a dilution of 1:100-1:400.
  
  Immunoprecipitation: use 1-4ug/mg lysate.
  
  ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500
  
  These are recommended dilutions.
  
  Endusers should determine optimal dilutions for their applications.
• Storage Conditions: This antibody is stable for at least one (1) year at 2-8°C.
• Specificity: Antibody was affinity-purified on GST.
• Formulation: Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Buffer pH: pH 7.2
• Target Background: A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.