Human NSE Protein, His Tag (MALS verified)

• Background:

  Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. It is also known as gamma-enolase or nolase 2 (ENO2) . It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. In clinical, NSE could be used as a biomarker for neuronal injury.

• Description:

  Human NSE, His Tag (NSE-H5144) is expressed from E. coli cells. It contains AA Met 1 - Leu 434 (Accession # P09104-1) .

• Specifications:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 49.3 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE) .

• Accession Number:

  P09104-1

• Expression Region:

  Met 1 - Leu 434

• Host:

  E. coli

• Target:

  NSE

• Conjugation:

  Unconjugated

• Tag:

  N-10xHis

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human NSE, His Tag (Cat. No. NSE-H5144) at 1 μg/mL (100 μL/well) can bind Anti-NSE antibody with a linear range of 1-63 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  20 mM Tris, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  49.3 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Human