Recombinant Xenopus tropicalis Hyaluronan synthase 1 (has1) , partial

Recombinant Xenopus tropicalis Hyaluronan synthase 1 (has1) , partial

• CAS Number: 9000-83-3
• Gene Name: has1
• UniProt: B1WB39
• Expression Region: 82-401aa
• Organism: Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
• Target Sequence: AYLEIRRINKTDLPCSFKKTVALTIAGYQENPDYLKHCLDSCRYVKYPKDKLKIILVIDGNTEDDAYMMEMFKDVFHGDDVGTYVWKGNYHTGVKETQDGSCPEVSKPLNEDEGIRIVEELVRTKRCVCIMQQWGGKREVMYTAFRAIGTTMDYVQVCDSDTKLDELATVEMVKVLEANELCGAVGGDVRILNPYDSFISFMSSLRYWMAFNVERACQSYFDCVSCISGPLGMYRNDILQVFLEAWHSQKFLGTYCTLGDDRHLTNRVLSMGYRTKYTPKCRAFSETPSQYLRWLNQQTRWTKSYFREWLYNAQWWYKHH
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Source: E.coli
• Field of Research: Others
• Assay Type: In Stock Protein
• Relevance: Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Partial
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 44.9 kDa
• References & Citations: NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2008)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.