Nesfatin-1/Nucleobindin-2 Polyclonal Antibody

• Background:

  Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1- 24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328) . Intracellular uncleaved NUCB2 is 45-50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420) . Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

• Abbreviation:

  Nesfatin-1/Nucleobindin-2

• UniProt:

  P80303

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Recombinant Human Nesfatin-1/Nucleobindin-2 protein expressed by E.coli

• Target:

  DNA-binding protein NEFA; NEFA; NEFAGastric cancer antigen Zg4; Nesfatin1; Nesfatin-1; NUCB2; nucleobindin 2; Nucleobindin-2; nucleobinding 2

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  IHC

• Field of Research:

  Signal Transduction; Epigenetics and Nuclear Signaling

• Purification:

  Antigen Affinity Purification

• Concentration:

  1 mg/mL

• Dilution:

  IHC 1:500-1:1000

• Buffer:

  PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

• Isotype:

  IgG