Rabbit anti Human C1q

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAT:

579-RAHu/C1q

Size:

1 mL

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Rabbit anti Human C1q

Background: The reactivity of the antiserum is restricted to C1q. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal Human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C1q protein. Complexes containing C1q may also react with the antiserum. No reaction is obtained with C1r, C1s or any other plasma protein component or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C1q or C1q containing complexes in Human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive nonprecipitating antibody-binding techniques if proper controls are included.
UniProt: P02746
Host: Rabbit
Clone: Polyclonal
Source: C1 component of Human complement is a complex of three glycoproteins: C1q, C1r and C1s. C1q has a molecular weight of 492.000 and is present in plasma in an average concentration of 80 μg/mL. It consists of a complex of 18 polypeptide chains of three different types, A, B and C; 6 of each type. The subcomponent C1q is responsible for the binding of C1q to immunocomplexes an membranes via the Fc portion of the immunoglobulin. The subunit C1q is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Assay Principle: Precipitation assays.
Form: Delipidated, heat inactivated lyophilized stable whole antiserum; No preservative added; Total protein and IgG concentrations in the antiserum are comparable to those of pooled; Normal rabbit serum; No foreign proteins added._x000D_ Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water
Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Storage Conditions: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the antiserum Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day

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