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Recombinant Human Plexin-B1 (PLXNB1), partial (Active)

CAT: 0399-CSB-MP018222HU2k6-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-MP018222HU2k6-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
(Semaphorin receptor SEP)
Abbreviation
Recombinant Human PLXNB1 protein, partial (Active)
Gene Name
PLXNB1
UniProt
O43157
Expression Region
20-535aa
Organism
Homo sapiens (Human)
Target Sequence
LQPLPPTAFTPNGTYLQHLARDPTSGTLYLGATNFLFQLSPGLQLEATVSTGPVLDSRDCLPPVMPDECPQAQPTNNPNQLLLVSPGALVVCGSVHQGVCEQRRLGQLEQLLLRPERPGDTQYVAANDPAVSTVGLVAQGLAGEPLLFVGRGYTSRGVGGGIPPITTRALWPPDPQAAFSYEETAKLAVGRLSEYSHHFVSAFARGASAYFLFLRRDLQAQSRAFRAYVSRVCLRDQHYYSYVELPLACEGGRYGLIQAAAVATSREVAHGEVLFAAFSSAAPPTVGRPPSAAAGASGASALCAFPLDEVDRLANRTRDACYTREGRAEDGTEVAYIEYDVNSDCAQLPVDTLDAYPCGSDHTPSPMASRVPLEATPILEWPGIQLTAVAVTMEDGHTIAFLGDSQGQLHRVYLGPGSDGHPYSTQSIQQGSAVSRDLTFDGTFEHLYVMTQSTLLKVPVASCAQHLDCASCLAHRDPYCGWCVLLGRCSRRSECSRGQGPEQWLWSFQPELGCLQ
Tag
N-terminal mFc-tagged
Type
Active Protein & In Stock Protein
Source
Mammalian cell
Field of Research
Neuroscience
Relevance
Receptor for SEMA4D (PubMed:19843518, PubMed:20877282, PubMed:21912513) . Plays a role in GABAergic synapse development. Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12196628, PubMed:15210733) . Plays a role in axon guidance, invasive growth and cell migration (PubMed:12198496) .
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D (CSB-MP835707HUd9) at 5 μg/mL can bind human PLXNB1, the EC50 is 0.8179-1.357 μg/mL.
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
83.2 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial

UniProtKB · O43157

Plexin-B1

PLXB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O43157
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PLXNB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A6H8Y2, Q6NY20, Q9UIV7, Q9UJ92, Q9UJ93
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

Domain

Coiled coilRepeatSignalTransmembraneTransmembrane helix

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

Receptor

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