Recombinant Chlamydophila felis tRNA uridine (34) hydroxylase (trhO)

• Product Name Alternative:

  (34) (tRNA hydroxylation protein O)

• Abbreviation:

  Recombinant Chlamydia felis trhO protein

• Gene Name:

  TrhO

• UniProt:

  Q252M0

• Expression Region:

  1-325aa

• Organism:

  Chlamydia felis (strain Fe/C-56) (Chlamydophila felis)

• Target Sequence:

  MKKNYYALAYYHFTRVDNPQEEIALHKELFKKLDVSCRIYISEQGINGQFSGYQPDAEYYMNWLKQRPGFSNVKFKIHHIEENIFPRATVKYRKELVALGCDVDLSNQGKHISPKEWHEKLEENRCLVLDVRNNYEWKIGHFENAVLPDIQTFREFPEYAEQLSKEHDPETTPVMMYCTGGIRCELYSSLLLEKGFKEVYQLDGGVIAYGQAMGTGKWRGKLFVFDDRLAVPIDEADTDVSPIAQCSHCEASCDTYYNCANTDCNNLFICCEECIHSTKGCCSQECSQAPRIRSFSTSRGNKPFRRMHLCEISEEQEKPLSCCLR

• Tag:

  N-terminal 6xHis-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Catalyzes oxygen-dependent 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  43.6 kDa

• References & Citations:

  "Genome sequence of the cat pathogen, Chlamydophila felis." Azuma Y., Hirakawa H., Yamashita A., Cai Y., Rahman M.A., Suzuki H., Mitaku S., Toh H., Goto S., Murakami T., Sugi K., Hayashi H., Fukushi H., Hattori M., Kuhara S., Shirai M. DNA Res. 13:15-23 (2006)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length