Rat HDL (High Density Lipoprotein) ELISA Kit

Rat HDL (High Density Lipoprotein) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HDL in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: High Density Lipoprotein
• Field of Research: Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 500 µg/mL
• Sample Type: Serum, plasma and other biological fluids.
• Detection Range: 7.82-500 µg/mL
• Sensitivity: 2.69 µg/mL