PCMV6-UBR5-3*FLAG
CAT: 1137-BZ68201Size: 1 VialDry Ice: NoHazardous: No
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Protein Expression
Storage Temperature
37°C
AA Sequence
MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLNSHPPLNVLEQATIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELGKPDNNDGSKLNSNSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRLGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPNLDKKNTPVQSPVSLGEDLQWWPDKDGTKFICIGALYSELLAVSSKGELYQWKWSESEPYRNAQNPSLHHPRATFLGLTNEKIVLLSANSIRATVATENNKVATWVDETLSSVASKLEHTAQTYSELQGERIVSLHCCALYTCAQLENSLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNMEKASKTTEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEEKVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSSNTNCQNSSGPDADPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGNWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLINLPANSTIKKKAAVIIMAVEKQTLMQHILRCDYEACRQYLMNLEQAVVLEQNLQMLQTFISHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTAATGTGQGPSTSTIPGPSTEPSVVESKDRKANAHFILKLLCDSVVLQPYLRELLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEISSSEKEEDVFMGMVCPSGTNPDDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTATNLVTLPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKTASPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGTIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAIAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSSSSSQSQSSYIIRNPQQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENAEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDIEQETFMLDEPLERTTNSSHANGAAQAPRSMQWAVRNTQHQRAASTAPSSTSTPAASSAGLIYIDPSNLRRSGTISTSAAAAAAALEASNASSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLTYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALASAGDPGHPNHPLHASQNSARRERMTAREEASLRTLEGRRRATLLSARQGMMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGIDNEDSEHENDDDTNQSATLNDKDDDSLPAETGQNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSSASSGKCLMEVTVDRNCLEVLPTKMSYAANLKNVMNMQNRQKKEGEEQPVLPEETESSKPGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLSLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAIAQAFLSNEKLPNLECIQNANKGTHTSLMQRLRNRGERDREREREREMRRSSGLRAGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPEPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV
Reference Sequence ID
NM_015902.5
Plasmid Hos
Mammalian cell
Inserted Fragment Type
ORF
Inserted Fragment Species
Human
Resistance
Kan
Selection antibiotic
G418
Competent cells
DH5a
Nucleic Acid Sequence
ATGACGTCCATCCATTTCGTGGTTCACCCGCTGCCGGGCACCGAGGACCAGCTCAATGACAGGTTACGAGAAGTTTCTGAGAAGCTGAACAAATATAATTTAAACAGCCACCCCCCTTTGAATGTATTGGAACAGGCTACTATTAAACAGTGTGTGGTGGGACCAAATCATGCTGCCTTTCTTCTTGAGGATGGTAGAGTTTGCAGGATTGGTTTTTCAGTACAGCCAGACAGATTGGAATTGGGTAAACCTGATAATAATGATGGGTCAAAGTTGAACAGCAACTCGGGGGCAGGGAGGACGTCAAGGCCTGGTAGGACAAGCGACTCTCCATGGTTTCTCTCAGGTTCTGAGACTCTAGGCAGGCTGGCAGGCAACACCTTAGGAAGCCGCTGGAGTTCTGGAGTGGGTGGAAGTGGTGGAGGATCCTCTGGTAGGTCATCAGCTGGAGCTCGAGATTCCCGCCGGCAGACTCGAGTTATTCGGACAGGACGGGATCGAGGGTCTGGGCTTTTGGGCAGTCAGCCCCAGCCAGTTATTCCAGCATCTGTCATTCCAGAGGAGCTGATTTCACAGGCCCAAGTTGTTTTACAAGGCAAATCCAGAAGTGTCATTATTCGAGAACTTCAGAGAACAAATCTTGATGTGAACCTTGCTGTAAATAATTTACTTAGCCGGGATGATGAAGATGGAGATGATGGGGATGATACAGCCAGCGAATCTTATTTGCCTGGAGAGGATCTTATGTCTCTCCTTGATGCCGACATTCATTCTGCCCACCCAAGTGTCATTATTGATGCAGATGCCATGTTTTCTGAAGACATTAGCTATTTTGGTTACCCTTCTTTTCGTCGTTCATCACTTTCCAGGCTAGGCTCATCTCGAGTTCTCCTTCTTCCCTTAGAGAGAGACTCTGAGCTGTTGCGTGAACGTGAATCCGTTTTACGTTTACGTGAACGAAGGTGGCTTGATGGAGCCTCATTTGATAATGAAAGGGGTTCTACCAGCAAGGAAGGAGAGCCAAACTTGGATAAGAAGAATACACCTGTTCAAAGTCCAGTATCTCTAGGAGAAGATTTGCAGTGGTGGCCTGATAAGGATGGAACAAAATTCATCTGTATTGGGGCTCTGTATTCTGAACTTCTGGCTGTCAGCAGTAAAGGAGAACTTTATCAGTGGAAATGGAGTGAATCTGAGCCTTACAGAAATGCCCAGAATCCTTCATTACATCATCCACGAGCAACATTTTTGGGGTTAACCAATGAAAAGATAGTCCTCCTGTCTGCAAATAGCATAAGAGCAACTGTAGCTACAGAAAATAACAAGGTTGCTACATGGGTGGATGAAACTTTAAGTTCTGTGGCTTCTAAATTAGAGCACACTGCTCAGACTTACTCTGAACTTCAAGGAGAGCGGATAGTTTCTTTACATTGCTGTGCCCTTTACACCTGCGCTCAGCTGGAAAACAGTTTATATTGGTGGGGTGTAGTTCCTTTTAGTCAAAGGAAGAAAATGTTAGAGAAAGCTAGAGCAAAAAATAAAAAGCCTAAATCCAGTGCTGGTATTTCTTCAATGCCGAACATCACTGTTGGTACCCAGGTATGCTTGAGAAATAATCCTCTTTATCATGCTGGAGCAGTTGCATTTTCAATTAGTGCTGGGATTCCTAAAGTTGGTGTCTTAATGGAGTCAGTTTGGAATATGAATGACAGCTGTAGATTTCAACTTAGATCTCCTGAAAGCTTGAAAAACATGGAAAAAGCTAGCAAAACTACTGAAGCTAAGCCTGAAAGTAAGCAGGAGCCAGTGAAAACAGAAATGGGTCCTCCACCATCTCCAGCATCCACGTGTAGTGATGCATCCTCAATTGCCAGCAGTGCATCAATGCCATACAAACGACGACGGTCAACCCCTGCACCAAAAGAAGAGGAAAAGGTGAATGAAGAGCAGTGGTCTCTTCGGGAAGTGGTTTTTGTGGAAGATGTCAAGAATGTTCCTGTTGGCAAGGTGCTAAAAGTAGATGGTGCCTATGTTGCTGTAAAATTTCCAGGAACCTCCAGTAATACTAACTGTCAGAACAGCTCTGGTCCAGATGCTGACCCTTCTTCTCTCCTGCAGGATTGTAGGTTACTTAGAATTGATGAATTGCAGGTTGTCAAAACTGGTGGAACACCGAAGGTTCCCGACTGTTTCCAAAGGACTCCTAAAAAGCTTTGTATACCTGAAAAAACAGAAATATTAGCAGTGAATGTAGATTCCAAAGGTGTTCATGCTGTTCTGAAGACTGGAAATTGGGTACGATACTGTATCTTTGATCTTGCTACAGGAAAAGCAGAACAGGAAAATAATTTTCCTACAAGCAGCATTGCTTTCCTTGGTCAGAATGAGAGGAATGTAGCCATTTTCACTGCTGGACAGGAATCTCCCATTATTCTTCGAGATGGAAATGGTACCATCTACCCAATGGCCAAAGATTGCATGGGAGGAATAAGGGATCCCGATTGGCTGGATCTTCCACCTATTAGTAGTCTTGGAATGGGTGTGCATTCTTTAATAAATCTTCCTGCCAATTCAACAATCAAAAAGAAAGCTGCTGTTATCATCATGGCTGTAGAGAAACAAACCTTAATGCAACACATTCTGCGCTGTGACTATGAGGCCTGTCGACAATATCTAATGAATCTTGAGCAAGCGGTTGTTTTAGAGCAGAATCTACAGATGCTGCAGACATTCATCAGCCACAGATGTGATGGAAATCGAAATATTTTGCATGCTTGTGTATCAGTTTGCTTTCCAACCAGCAATAAAGAAACTAAAGAAGAAGAGGAAGCGGAGCGTTCTGAAAGAAATACATTTGCAGAAAGGCTTTCTGCTGTTGAGGCCATTGCAAATGCAATATCAGTTGTTTCAAGTAATGGCCCAGGTAATCGGGCTGGATCATCAAGTAGCCGAAGTTTGAGATTACGGGAAATGATGAGACGTTCGTTGAGAGCAGCTGGTTTGGGTAGACATGAAGCTGGAGCTTCATCCAGTGACCACCAGGATCCAGTTTCACCCCCCATAGCTCCCCCTAGTTGGGTTCCTGACCCTCCTGCGATGGATCCTGATGGTGACATTGATTTTATCCTGGCCCCCGCTGTGGGATCTCTTACCACAGCAGCAACCGGTACTGGTCAAGGACCAAGCACCTCCACTATTCCAGGTCCTTCCACAGAGCCATCTGTAGTAGAATCCAAGGATCGAAAGGCGAATGCTCATTTTATATTGAAATTGTTATGTGACAGTGTGGTTCTCCAGCCCTATCTACGAGAACTTCTTTCTGCCAAGGATGCAAGAGGGATGACCCCATTTATGTCAGCTGTAAGTGGCCGAGCTTATCCTGCTGCAATTACCATCTTAGAAACTGCTCAGAAAATTGCAAAAGCTGAAATATCCTCAAGTGAAAAAGAGGAAGATGTATTCATGGGAATGGTTTGCCCATCAGGTACCAACCCTGATGACTCTCCTTTATATGTTTTATGTTGTAATGACACTTGCAGTTTTACATGGACTGGAGCAGAGCACATTAACCAGGATATTTTTGAGTGTCGAACTTGTGGCTTGCTGGAGTCACTGTGTTGTTGTACGGAATGTGCAAGGGTTTGTCATAAAGGTCATGATTGCAAACTCAAACGGACATCACCAACAGCCTACTGTGATTGTTGGGAGAAATGTAAATGTAAAACTCTTATTGCTGGACAGAAATCTGCTCGTCTTGATCTACTTTATCGCCTGCTCACTGCTACTAATCTGGTTACTCTGCCAAACAGCAGGGGAGAGCACCTCTTACTATTCTTAGTACAGACAGTCGCAAGGCAGACGGTGGAGCATTGTCAATACAGGCCACCTCGAATCAGGGAAGATCGTAACCGAAAAACAGCCAGTCCTGAAGATTCAGATATGCCAGATCATGATTTAGAGCCTCCAAGATTTGCCCAGCTTGCATTGGAGCGTGTTCTACAGGACTGGAATGCCTTGAAATCTATGATTATGTTTGGGTCGCAGGAGAATAAAGACCCTCTTAGTGCCAGCAGTAGAATAGGCCATCTTTTGCCAGAAGAGCAAGTATACCTCAATCAGCAAAGTGGCACAATTCGGCTGGACTGTTTCACTCATTGCCTTATAGTTAAGTGTACAGCAGATATTTTGCTTTTAGATACTCTACTAGGTACACTAGTGAAAGAACTCCAAAACAAATATACACCTGGACGTAGAGAAGAAGCTATTGCTGTGACAATGAGGTTTCTACGTTCAGTGGCAAGAGTTTTTGTTATTCTGAGTGTGGAAATGGCTTCATCCAAAAAGAAAAACAACTTTATTCCACAGCCAATTGGAAAATGCAAGCGTGTATTCCAAGCATTGCTACCTTACGCTGTGGAAGAATTGTGCAACGTAGCAGAGTCACTGATTGTTCCTGTCAGAATGGGGATTGCTCGTCCAACTGCACCATTTACCCTGGCTAGTACTAGCATAGATGCCATGCAGGGCAGTGAAGAATTATTTTCAGTGGAACCACTACCACCACGACCATCATCTGATCAGTCTAGCAGCTCCAGTCAGTCTCAGTCATCCTACATCATCAGGAATCCACAGCAGAGGCGCATCAGCCAGTCACAGCCCGTTCGGGGCAGAGATGAAGAACAGGATGATATTGTTTCAGCAGATGTGGAAGAGGTTGAGGTGGTGGAGGGTGTGGCTGGAGAAGAGGATCATCATGATGAACAGGAAGAACACGGGGAAGAAAATGCTGAGGCAGAGGGACAACATGATGAGCATGATGAAGACGGGAGTGATATGGAGCTGGACTTGTTAGCAGCAGCTGAAACAGAAAGTGATAGTGAAAGTAACCACAGCAACCAAGATAATGCTAGTGGGCGCAGAAGCGTTGTCACTGCAGCAACTGCTGGTTCAGAAGCAGGAGCAAGCAGTGTTCCTGCCTTCTTTTCTGAAGATGATTCTCAATCGAATGACTCAAGTGATTCTGATAGCAGTAGTAGTCAGAGTGACGACATAGAACAGGAGACCTTTATGCTTGATGAGCCATTAGAAAGAACCACAAATAGCTCCCATGCCAATGGTGCTGCCCAAGCTCCCCGTTCAATGCAGTGGGCTGTCCGCAACACCCAGCATCAGCGAGCAGCCAGTACAGCCCCTTCCAGTACATCTACACCAGCAGCAAGTTCAGCGGGTTTGATTTATATTGATCCTTCAAACTTACGCCGGAGTGGTACCATCAGTACAAGTGCTGCAGCTGCAGCAGCTGCTTTGGAAGCTAGCAACGCCAGCAGTTACCTAACATCTGCAAGCAGTTTAGCCAGGGCTTACAGCATTGTCATTAGACAAATCTCGGACTTGATGGGCCTTATTCCTAAGTATAATCACCTAGTATACTCTCAGATTCCAGCAGCTGTGAAATTGACTTACCAAGATGCAGTAAACTTACAGAACTATGTAGAAGAAAAGCTTATTCCCACTTGGAACTGGATGGTCAGTATTATGGATTCTACTGAAGCTCAATTACGTTATGGTTCTGCATTAGCATCTGCTGGTGATCCTGGACATCCAAATCATCCTCTTCACGCTTCTCAGAATTCAGCGAGAAGAGAGAGGATGACTGCGCGAGAAGAAGCTAGCTTACGAACACTTGAAGGCAGACGACGTGCCACCTTGCTTAGCGCCCGTCAAGGAATGATGTCTGCACGAGGAGACTTCCTAAATTATGCTCTGTCTCTAATGCGGTCTCATAATGATGAGCATTCTGATGTTCTTCCAGTTTTGGATGTTTGCTCATTGAAGCATGTGGCATATGTTTTTCAAGCACTTATATACTGGATTAAGGCAATGAATCAGCAGACAACATTGGATACACCTCAACTAGAACGCAAAAGGACGCGAGAACTCTTGGAACTGGGTATTGATAATGAAGATTCAGAACATGAAAATGATGATGACACCAATCAAAGTGCTACTTTGAATGATAAGGATGATGACTCTCTTCCTGCAGAAACTGGCCAAAACCATCCATTTTTCCGACGTTCAGACTCCATGACATTCCTTGGGTGTATACCCCCAAATCCATTTGAAGTGCCTCTGGCTGAAGCCATCCCCTTGGCTGATCAGCCACATCTGTTGCAGCCAAATGCTAGAAAGGAGGATCTTTTTGGCCGTCCAAGTCAGGGTCTTTATTCTTCATCTGCCAGTAGTGGGAAATGTTTAATGGAGGTTACAGTGGATAGAAACTGCCTAGAGGTTCTTCCAACAAAAATGTCTTATGCTGCCAATCTGAAAAATGTAATGAACATGCAAAACCGGCAAAAAAAAGAAGGGGAAGAACAGCCCGTGCTGCCAGAAGAAACTGAGAGTTCAAAACCAGGGCCATCTGCTCATGATCTTGCTGCACAATTAAAAAGTAGCTTACTAGCAGAAATAGGACTTACTGAAAGTGAAGGGCCACCTCTCACATCTTTCAGGCCACAGTGTAGCTTTATGGGAATGGTTATTTCCCATGATATGCTGCTAGGACGTTGGCGCCTTTCTTTAGAACTGTTCGGCAGGGTATTCATGGAAGATGTTGGAGCAGAACCTGGATCAATCCTAACTGAATTGGGTGGTTTTGAGGTAAAAGAATCAAAATTCCGCAGAGAAATGGAAAAACTGAGAAACCAGCAGTCAAGAGATTTGTCACTAGAGGTTGATCGGGATCGAGATCTTCTCATTCAGCAGACTATGAGGCAGCTTAACAATCACTTTGGTCGAAGATGTGCTACTACACCAATGGCTGTACACAGAGTAAAAGTCACATTTAAGGATGAGCCAGGAGAGGGCAGTGGTGTAGCACGAAGTTTTTATACAGCCATTGCACAAGCATTTTTATCAAATGAAAAATTGCCAAATCTAGAGTGTATCCAAAATGCCAACAAAGGCACCCACACAAGTTTAATGCAGAGATTAAGGAACCGAGGAGAGAGAGACCGGGAAAGGGAGAGAGAAAGGGAAATGAGGAGGAGTAGTGGTTTGCGAGCAGGTTCTCGGAGGGACCGGGATAGAGACTTTAGAAGACAGCTTTCCATCGACACTAGGCCCTTTAGACCAGCCTCTGAAGGGAATCCTAGCGATGATCCTGAGCCTTTGCCAGCACATCGGCAGGCACTTGGAGAGAGGCTTTATCCTCGTGTACAAGCAATGCAACCAGCATTTGCAAGTAAAATCACTGGCATGTTGTTGGAATTATCCCCAGCTCAGCTGCTTCTCCTTCTAGCAAGTGAGGATTCTCTGAGAGCAAGAGTGGATGAGGCCATGGAACTCATTATTGCACATGGACGGGAAAATGGAGCTGATAGTATCCTGGATCTTGGATTAGTAGACTCCTCAGAAAAGGTACAGCAGGAAAACCGAAAGCGCCATGGCTCTAGTCGAAGTGTAGTAGATATGGATTTAGATGATACAGATGATGGTGATGACAATGCCCCTTTGTTTTACCAACCTGGGAAAAGAGGATTTTATACTCCAAGGCCTGGCAAGAACACAGAAGCAAGGTTGAATTGTTTCAGAAACATTGGCAGGATTCTTGGACTATGTCTGTTACAGAATGAACTATGTCCTATCACATTGAATAGACATGTAATTAAAGTATTGCTTGGTAGAAAAGTCAATTGGCATGATTTTGCTTTTTTTGATCCTGTAATGTATGAGAGTTTGCGGCAACTAATCCTCGCGTCTCAGAGTTCAGATGCTGATGCTGTTTTCTCAGCAATGGATTTGGCATTTGCAATTGACCTGTGTAAAGAAGAAGGTGGAGGACAGGTTGAACTCATTCCTAATGGTGTAAATATACCAGTCACTCCACAGAATGTATATGAGTATGTGCGGAAATACGCAGAACACAGAATGTTGGTAGTTGCAGAACAGCCCTTACATGCAATGAGGAAAGGTCTACTAGATGTGCTTCCAAAAAATTCATTAGAAGATTTAACGGCAGAAGATTTTAGGCTTTTGGTAAATGGCTGCGGTGAAGTCAATGTGCAAATGCTGATCAGTTTTACCTCTTTCAATGATGAATCAGGAGAAAATGCTGAGAAGCTTCTGCAGTTCAAGCGTTGGTTCTGGTCAATAGTAGAGAAGATGAGCATGACAGAACGACAAGATCTTGTTTACTTTTGGACATCAAGCCCATCACTGCCAGCCAGTGAAGAAGGATTCCAGCCTATGCCCTCAATCACAATAAGACCACCAGATGACCAACATCTTCCTACTGCAAATACTTGCATTTCTCGACTTTACGTCCCACTCTATTCCTCTAAACAGATTCTCAAACAGAAATTGTTACTCGCCATTAAGACCAAGAATTTTGGTTTTGTGTAG
Gene Length
8400
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