Multiplex IHC Detection Kit (Quintuple)

CAT: 1601-CRG1088-01Size: 20 AssaysDry Ice: NoHazardous: No
CAT#:1601-CRG1088-01Size:20 Assays
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Five colors multiplex IHC tyramide signal amplification (TSA)
Applications
MIHC, IF
Storage Conditions
Store at 4 °C in dark for 1 year, do not freeze.
Notes
Tyramide signal amplification (TSA), also called catalyzed reporter deposition (CARD), is a highly sensitive enzymatic method which can enable the detection of low-abundance targets in histochemical analysis. TSA utilizes the catalytic activity of HRP for the covalent deposition of labeled tyramide on and near target proteins or nucleic acid sequences in situ. In the presence of low hydrogen peroxide (H2O2), HRP is able to convert labeled tyramide substrates into highly-reactive, short-lived tyramide radicals that rapidly bind to tyrosine residues on and proximal to the enzyme site. These labels can be detected by standard chromogenic or fluorescent techniques. Multiple rounds of tyramide signal amplification can be performed for multicolor detection.

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