Cycle Pure Kit

CAT: 0853-DC3514-00Size: 4 / BoxDry Ice: NoHazardous: No
CAT#:0853-DC3514-00Size:4 / Box
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Cycle Pure Kit is a rapid DNA clean-up system designed to purify single- or double-stranded DNA fragments derived from PCR or other enzymatic reactions. Starting with small reaction volumes (typically 5–50 μL), samples are mixed with a binding buffer and applied to a silica-based spin column. After two wash steps, the purified DNA is eluted in 30–50 μL of nuclease-free water or low-salt buffer. The system efficiently recovers DNA fragments from ~100 bp up to ~10 kb (or more) with yields exceeding ~80–90% under optimal conditions. The eluate is free of primers, nucleotides, enzymes and salts, making it immediately suitable for downstream applications such as T-A ligation, restriction enzyme digestion, sequencing, cloning or labeling reactions. No phenol/chloroform extraction or ethanol precipitation is required, and total processing time is under ~10 minutes. The kit includes spin columns, collection tubes, binding buffer, wash buffer (s), elution buffer and a detailed user protocol. Ideal for molecular biology labs, cloning workflows and high-throughput PCR clean-up.
Hazard Statement
N
Shipping Conditions
Ambient
Shelf Life
24
Latex Free Indicator
Latex Free

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