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Ubiquitin aldehyde, (recombinant)

CAT: 1106-BML-UW8450-0050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:1106-BML-UW8450-0050Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
Ki=2.5nM vs. UCH isopeptidase-T.
Product Name Alternative
Ub-H
Certification
RUO
UniProt
P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
Source
Produced in E.coli.
Sequence
Recombinant Ubiquitin (corresponding to UniProt sequence P62988) . The C-terminal glycine carboxyl is synthetically modified to an aldehyde.
Purity
≥95% (HPLC)
Molecular Weight
8.5kDa
Shipping Conditions
Blue Ice
Storage Conditions
+4°C
Notes
Typical assay set-up: Substrate concentration: 0.01-1.0μM. Enzyme concentration, UCH-L1 (human) (recombinant) (His) (BML-UW9740) : 10-100nM. Inhibitor concentration: 0.01-1.0μM. Release of AMC fluorescence by DUB enzymes can be monitored using 380nm excitation and 460nm emission wavelengths.
Formulation
Liquid. In aqueous solution containing 0.15M HCl.
Applications Notes
Ubiquitin Aldehyde (recombinant) is useful in the stabilisation of ubiquitin-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts. Inhibition of deubiquitinylating enzyme activity by Ubiquitin Aldehyde (recombinant) can be used to identify and confirm such activity and to determine the inhibition kinetics for a particular enzyme. Recommended concentration for maximal inhibition is 2-5μM. Co-crystallisation of ubiquitin aldehyde with specific deubiquitinylating enzymes (the inhibitor mimics the natural ubiquitin substrate) has also been used to probe enzyme:substrate interactions.
Quality Control
Ubiquitin Aldehyde (recombinant) activity was confirmed by its inhibition of human UCH-L1 and deubiquitinylation of Ubiquitin-AMC (Prod. No. BML-SE211) .

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