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Ubiquitin activating kit

CAT: 1106-BML-UW0400A-0001Size: 20 TestsDry Ice: YesHazardous: No
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CAT#:1106-BML-UW0400A-0001Size:20 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Certification
RUO
UniProt
P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Applications Notes
Uses: Activation of Ubiquitin for conjugation to wild type or mutant E2 enzymes (user supplied) via thioester bond formation. Use of Ub-E2 conjugates produced in the subsequent ubiquitin modification of specific target proteins in presence of a dedicated ubiquitin E3 ligase. Investigation of ubiquitin activation by E1 activating enzyme. Substitution of the wild type ubiquitin provided with ubiquitin mutants or derivatives (e.g. biotinylated-ubiquitin, Prod. No. BML-UW8705) allowing their activation and subsequent utility in the ubiquitin cascade.
Contents
20X Ubiquitin Activating Enzyme Solution (E1) 20X Ubiquitin Solution (Ub) Ubiquitin Antibody Solution 20X Mg-ATP Solution 2X Non-reducing Gel Loading Buffer 10X Ubiquitinylation Buffer

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