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RsaI

CAT: 1106-ENZ-GEN109-1000Size: 1000 UDry Ice: NoHazardous: No
CAT#:1106-ENZ-GEN109-1000Size:1000 U
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PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Certification
RUO
Source
Produced in an E. coli strain that carries the cloned RsaI gene from Rhodopseudomonas sphaeroides .
Concentration
RsaI 10,000 units/mlIncludes 10X Cutting Buffer
Reconstitution
Reconstitute 10X Cutting Buffer with nuclease-free water
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Formulation
Liquid. In 100mM sodium chloride, containing 10mM Tris-HCl, pH 7.4, 1mM DTT, 0.1mM EDTA, 50% glycerol and 200μg/ml BSA. After reconstitution, 1X Cutting Buffer: 50mM potassium acetate, 20mM Tris-acetate, 10mM magnesium acetate, 100μg/ml BSA, pH 7.9, at 25°C.
Applications Notes
For molecular cloning, restriction site mapping, genotyping, Southern blotting, SNP.
Quality Control
Exonuclease Activity (Radioactivity Release) : A 50 μL reaction in 1X Cutting Buffer containing 1 μg of a mixture of single and double stranded [ 3 H] E. coli DNA and a minimum of 50 units of RsaI incubated for 4 hours at 37°C releases <0.1% of the total radioactivity. Ligation and Recutting (Terminal Integrity) : After a 10-fold over digestion of λ DNA with RsaI, ~95% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16°C. Of these ligated fragments, >95% can be recut with RsaI. Non-Specific DNase Activity (16 Hour) : A 50 μL reaction in 1X Cutting Buffer containing 1 μg of λ DNA and a minimum of 50 Units of RsaI incubated for 16 hours at 37°C results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.

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