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NUCLEAR-ID®Green cell cycle kit

CAT: 1106-ENZ-51014-100Size: 1 KitDry Ice: YesHazardous: No
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CAT#:1106-ENZ-51014-100Size:1 Kit
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Certification
RUO
Applications
Flow Cytometry, Fluorescence microscopy
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Notes
The NUCLEAR-ID ® Green cell cycle analysis kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required .
Applications Notes
This kit is suitable for DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels.
Quality Control
Absorption peak of NUCLEAR-ID ® Green dye: λ max = 507 ± 4 nm % purity of NUCLEAR-ID ® Green dye by HPLC: ≥93% A sample from each lot of NUCLEAR-ID ® Green Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. Cells with Nocodazole gave %G 2 value of >50%. Untreated cells gave the following results: (a) G 0 /G 1 peak CV < 15%; (b) %G 1 > 35%; (c) %G 2 < 30%; and (d) G 2 /G 1 ratio > 1.72.
Contents
NUCLEAR-ID ® Green Cell Cycle Detection Reagent, 100 μl Nocodazole Control, 10 μl 10X Assay Buffer, 15 ml

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