Neutrophil elastase (human), (purified)

CAT: 1106-BML-SE284-0100Size: 100 µgDry Ice: YesHazardous: No

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CAT#:1106-BML-SE284-0100Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Specifications

20-22 U/mg protein. One unit will hydrolyze 1.0 μmole of MeOSuc-Ala-Ala-Pro-Val-pNA (Prod. No. BML-P213) per minute, at 25°C in 100mM Tris-HCl, pH 7.5 with 500 mM NaCl.

Product Name Alternative

Leukocyte elastase, LE

Certification

RUO

UniProt

P08246

Source

Isolated from human neutrophils. Active enzyme.

Stability

As indicated on product label or CoA when stored as recommended. This enzyme is stable when stored under the above conditions. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity.

Purity

≥95% (SDS-PAGE) . Although this is in highly purified form, and the source material tested negative for HbsAg, anti-HBc, anti-HCV, and anti-HIV-1 and -2, handle this product with all due precautions.

Reconstitution

Reconstitute with 50mM sodium acetate, pH 5.5, with 150mM sodium chloride to the original volume, followed by gentle mixing to ensure adequate homogenization.

Shipping Conditions

Dry Ice

Storage Conditions

-20°C

Applications Notes

Useful for inhibitor screening, kinetic studies, and cleavage of substrates in vitro or in tissue culture.

UniProtKB · P08246

Neutrophil elastase

ELNE_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P08246
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ELANE
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 3.4.21.37
Processing: Precursor
Secondary accessions: P09649, Q6B0D9, Q6LDP5

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cytoplasmic vesicleLysosomeNucleus

Disease

Disease variant

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Domain

Signal

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Alternative Products

Catalog	Name
MBS566608-01	Neutrophil Elastase (human) (purified)
ETSE15-N-100	Elastase, Human Neutrophil, purified
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IHUELASD1MG	Human Neutrophil Elastase Purified Lyophilized 1mg
IHUELASD100UG	Human Neutrophil Elastase Purified Lyophilized 100ug
MBS5308232-01	Purified native Human Neutrophil Elastase protein
ETSE11-C	Purified Human Neutrophil Elastase protein control for WB
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GTR19320342	HUMAN NEUTROPHIL ELASTASE
T89176-01	Neutrophil Elastase, Human