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Nerve growth factor 7S (mouse)

CAT: 1106-ALX-521-008-C100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1106-ALX-521-008-C100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
NGF 7S
Certification
RUO
UniProt
P01139
Source
Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4 , 79 (1984) .
Stability
As indicated on product label or CoA when stored as recommended. Stable for 2-3 weeks when stored at room temperature. After reconstitution, store for up to one week at +4°C and for 4-6 weeks at -80°C.
Purity
≥98% (HPLC)
Activity
Bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Neurotrophic activity is in the range of 0.5-50ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100ng/ml medium.
Reconstitution
Dilute in sterile water or DMEM to a concentration of at least 10μg/ml and store at -80°C before use. Medium containing NGF cannot be reused due to NGF degradation.
Molecular Weight
~130kDa.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Formulation
Lyophilized.

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