Mouse Interferon Beta, mammalian

CAT: 1106-PBL-12405-1Size: 0.1 MUDry Ice: YesHazardous: No
CAT#:1106-PBL-12405-1Size:0.1 MU
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Interferon-β, IFN-β
Certification
RUO
Source
Gene was obtained from mouse DNA expressed in mammalian cells
Purification
Purified from mammalian tissue culture media by a combination of ion exchange, hydrophobic interaction and size exclusion chromatography.
Stability
After receipt, this product should be kept at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed – the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA) .
Endotoxin
< 1 EU/μg
Purity
≥95%
Activity
Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as listed. Mouse (L929/EMCV) -performed as described [Rubinstein et al. (1981) J. Virol. 37 (2) :755]. The EC50 for interferon in this assay is ~ 2.5 U/ml. Lot Activity was derived from multiple determinations in the above assay. The units are determined by use of a Mu IFN-β laboratory standard calibrated to the international reference standard for mouse Interferon Beta provided by the National Institutes of Health [Meager et al. (2001) J. Immunol Methods. 257 (1-2) :17-33]. Please note that IFN assays vary between labs and assay systems [Meager et al (2001) . J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Molecular Weight
~19.6kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Appearance
Liquid
Formulation
Liquid. 20 mM Sodium Phosphate, pH 6.4; 300 mM NaCl; 5% Glycerol, 0.1% bovine serum albumin (BSA) .

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