C2 Ceramide

CAT: 1106-BML-SL100-0005Size: 5 mgDry Ice: NoHazardous: No
CAT#:1106-BML-SL100-0005Size:5 mg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
3102-57-6
Product Name Alternative
N-Acetyl-D-erythro-sphingosine
Certification
RUO
Target
Jnk, MAPK, Serine/threonine-protein phosphatase
Type
Activator
Stability
As indicated on product label or CoA when stored as recommended. Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C.
Purity
≥98% (TLC)
Solubility
Soluble in 100% ethanol (>25mg/ml) or DMSO (>50mg/ml) .
Molecular Formula
C 20 H 39 NO 3
Molecular Weight
341.5
Shipping Conditions
Ambient Temperature
Storage Conditions
-20°C
Notes
C2 ceramide may precipitate from aqueous media, particularly those lacking serum or serum albumin, at concentrations ~20μM or higher. To avoid this problem, a 1:1 complex of C2 Ceramide with BSA may be prepared as follows: Dissolve C2 ceramide to 200mM (68.3mg/ml) in anhydrous DMSO. Dissolve fatty acid-free BSA in water to 66mg/ml (1mM) . Dilute the ceramide/DMSO stock 200-fold into the BSA solution, with constant stirring. A precipitate will form at first, but will dissolve after ~30 minutes of stirring at room temperature. Alternatively, a solution of C2 Ceramide/BSA may be prepared directly, without making a DMSO stock: 1) prepare a 20% w/v stock of BSA in water (3mM) ; 2) use the BSA stock to dissolve the solid C2 Ceramide at 1.02mg/ml (3mM) . Complete dissolution of the ceramide may require more than 1 hour of stirring at room temperature.
Appearance
White solid.

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