EIF2α (AB1973) Rabbit mAb

• Background :

  The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al. , 1987 [PubMed 2948954]) . [supplied by OMIM, Feb 2010]

• Synonyms :

  EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha

• Gene Name :

  EIF2S1

• Gene ID :

  1965

• Swiss Prot :

  P05198

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant protein (or fragment) . This information is considered to be commercially sensitive.

• Clonality :

  Monoclonal

• Source :

  Monoclonal, Rabbit, IgG

• Applications :

  WB, IHC, IF, IP, ELISA

• Purification :

  The antibody was purified by protein A affinity chromatography.

• Dilution :

  WB, 1:2000-1:10000 | IHC, 1:1000-1:5000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

• Modification :

  Unmodified

• Storage Conditions :

  Store at-20°C. Avoid freeze / thaw cycles.

• Specificity :

  This antibody detects endogenous levels of eIF2α protein.

• Formulation :

  The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% Proclin 300.

• Protein Name :

  EIF2α