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Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

CAT: 0399-CSB-YP357716FKZ-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-YP357716FKZ-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Alpha-HL; Alpha-toxin
Abbreviation
Recombinant Staphylococcus aureus hly protein
Gene Name
Hly
UniProt
P09616
Expression Region
27-319aa
Organism
Staphylococcus aureus
Target Sequence
ADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYERVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN
Tag
Tag-Free
Type
In Stock Protein
Source
Yeast
Field of Research
Microbiology
Relevance
Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
34.0 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein

UniProtKB · P09616

Alpha-hemolysin

HLA_STAAU · Staphylococcus aureus

View on UniProt ↗
Primary accession
P09616
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
hly
Protein existence
1: Evidence at protein level
Organism
Staphylococcus aureus
Taxonomy ID
1280
Alternative names
Alpha-HL
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencing

Biological process

CytolysisHemolysisVirulence

Cellular component

Secreted

Domain

Signal

Molecular function

Toxin

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