Recombinant Lucilia sericata Lucifensin

• Product Name Alternative:

  (Sericasin)

• Abbreviation:

  Recombinant Lucilia sericata Lucifensin protein

• UniProt:

  P86471

• Expression Region:

  53-92aa

• Organism:

  Lucilia sericata (Green bottle fly) (Phaenicia sericata)

• Target Sequence:

  ATCDLLSGTGVKHSACAAHCLLRGNRGGYCNGRAICVCRN

• Tag:

  N-terminal 6xHis-KSI-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Shows strong antibacterial activity against numerous Gram-positive bacteria. It selectively inhibits peptidoglycan biosynthesis through complex formation with the cell wall precursor lipid II (1:1 molar ratio) thus inhibiting cell wall synthesis. Shows antibacterial activity against the Gram-positive bacteria M.luteus, E.fecalis (MIC=32 mg/L), S.aureus (MIC=16 mg/L), S.carnosus (MIC=2 mg/L), S.pneumoniae (MIC=2 mg/L) and S.pyogenes (MIC=2 mg/L) and against a number of methicillin-resistant S.aureus and glycopeptide-intermediate S.aureus isolates. Does not show antibacterial activity against Gram-negative bacteria or antifungal activity against C.utilis. Shows slight antifungal activity against C.albicans.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  19.5 kDa

• References & Citations:

  "Lucifensin, the long-sought antimicrobial factor of medicinal maggots of the blowfly Lucilia sericata." Cerovsky V., Zdarek J., Fucik V., Monincova L., Voburka Z., Bem R. Cell. Mol. Life Sci. 67:455-466 (2010)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein