EasyStep Rat T4 (Thyroxine) ELISA Kit

EasyStep Rat T4 (Thyroxine) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat T4 antigen, and the Rat T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
• Field of Research: Endocrinology; Hormone metabolism.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 1.5h
• Standard: 600 ng/mL
• Sample Type: Serum, plasma
• Detection Range: 9.38-600 ng/mL
• Sensitivity: 7 ng/mL