Human PGF2α (Prostaglandin F2 Alpha) ELISA Kit
CAT:
855-ELK10032-02
Size:
96 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Human PGF2α (Prostaglandin F2 Alpha) ELISA Kit
- Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
- CAS Number: 7732-18-5
- Gene Aliases: 8-isoprostane; 8-iso-PGF2α
- Field of Research: Metabolic pathway; Cardiovascular biology; Hormone metabolism.
- Assay Principle: Competitive Inhibition ELISA
- Assay Performance Time: 2.5h
- Standard: 800 pg/mL
- Sample Type: Serum, plasma, saliva and other biological fluids.
- Detection Range: 12.5-800 pg/mL
- Sensitivity: 3.65 pg/mL