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Anti-eEF2K (C-terminus) Antibody

CAT: 1549-EP4661Size: 100 µLDry Ice: NoHazardous: No
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CAT#:1549-EP4661Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 is an autophosphorylation site that is required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38delta causes inactivation of eEF2K. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.
Description
Our eEF2K (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and xenopus eEF2K (C-terminus) and is antigen affinity purified. It is great for use in ELISA, WB, ICC, IP.
Short Description
Our eEF2K (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus eEF2K (C-terminus) and is antigen affinity purified. It is great for use in WB, ICC, IP.
Synonyms
EEF-2, eEF-2K, CaMK-III, eukaryotic elongation factor
Gene Name
EEF2K
UniProt
O00418
Reactivity
Chicken, Human, Mouse, Rat, and Xenopus
Immunogen
EEF2K (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human eEF2K. This sequence is well conserved in rat, mouse, chicken, and fish eEF2K, and has low homology to other proteins.
Target
EEF2K (C-terminus)
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
ELISA, WB, ICC, IP
Field of Research
Apoptosis, Cell Signaling, Kinase Signaling, Epigenetics, Oncoproteins
Format
Antigen Affinity Purified
Buffer
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Molecular Weight
105
Precautions
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Shipping Conditions
Blue Ice
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Notes
Affinity purified rabbit serum
WB Dilution
1:1000
IP Dilution
1:100
Specificity
The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human A431, HeLa, and Jurkat cells. The antibody also detected eEF2K in immunoprecipitations in HeLa cells and immunofluorescent labeling in A431 cells.
ICC Dilution
1:200
Quality Control
Western blots performed on each lot.
Host or Source
Rabbit
Physical Properties
Liquid
Isotype
IgG
Immunogen Species
Human
WB Quality Control
Quality Control: WB
RRID
AB_3068433

UniProtKB · O00418

Eukaryotic elongation factor 2 kinase

EF2K_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O00418
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EEF2K
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
eEF-2 kinase, eEF-2K
EC number
2.7.11.20
Processing
—
Secondary accessions
Q8N588
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Ligand

ATP-bindingCalciumNucleotide-binding

Molecular function

Calmodulin-bindingKinaseSerine/threonine-protein kinaseTransferase

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