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HSP27 Mouse

CAT: 0793-32-13811-10Size: 10 µgDry Ice: NoHazardous: No
CAT#:0793-32-13811-10Size:10 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source:Escherichia Coli.Physical Appearance:Sterile Filtered colorless solution.Biological ActivitynullHSP27 or Estrogen-Regulated 24K proteinis a part of the mammalian small heat shock protein family. The protein is expressed constitutively in many tissues and its expression is increased to high levels after different types of stress including high temperatures, toxic metals, drugs and oxidants. Furthermore, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38) .HSP27 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 232 amino acids (1-209 a.a) and having a molecular mass of 25.4kDa. HSP27 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Name Alternative
HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.
Purification
Greater than 90.0% as determined by SDS-PAGE.
Format
The HSP27 protein solution (1mg/mL) contains 20mM Tris-HCl (pH8.0), 10% glycerol and 1mM DTT.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MGSMTERRVP FSLLRSPSWE PFRDWYPAHS RLFDQAFGVP RLPDEWSQWF SAAGWPGYVR PLPAATAEGP AAVTLAAPAF SRALNRQLSS GVSEIRQTAD RWRVSLDVNH FAPEELTVKT KEGVVEITGK HEERQDEHGY ISRCFTRKYT LPPGVDPTLV SSSLSPEGTL TVEAPLPKAV TQSAEITIPV TFEARAQIGG PEAGKSEQSG AK.

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