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PF4 Bovine

CAT: 0793-32-6613-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0793-32-6613-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source: Escherichia Coli.Sterile Filtered White lyophilized (freeze-dried) powder.Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets.PF4â€TMs major physiologic role appears to be neutralization of molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Furthermore, it is used as an inhibitor in the angiogenesis during tumor therapy.Platelet Factor-4 (CXCL4) Bovine Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 88 amino acid and having a molecular mass of approximately 9.5kDa.PF4 is purified by proprietary chromatographic techniques.
Product Name Alternative
CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
Applications
Functional Assay
Purification
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Components
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB and 500mM NaCl, pH 7.0.It is recommended to reconstitute the lyophilized Platelet Factor-4 in sterile 18M Omega -cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions
Lyophilized CXCL4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Platelet Factor-4 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Applications Notes
The biological activity determined by a chemotaxis bioassay using human neutrophils is 10-100ng/ml.
Amino Acids
ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDES.

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