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Recombinant Human SMARCA4

CAT: 0793-32-4868-20Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0793-32-4868-20Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. SMARCA4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (362-549aa) and having a molecular mass of 25kDa. SMARCA4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. SMARCA4 belongs to the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Proteins in this family have helicase and ATPase activities and are considered to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA4 is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is essential for transcriptional activation of genes normally repressed by chromatin. In addition, SMARCA4 is able to bind BRCA1, as well as regulating the expression of the tumorigenic protein CD44. Moreover, mutations in SMARCA4 cause rhabdoid tumor predisposition syndrome type 2.
Product Name Alternative
SWI/SNF Related||Matrix Associated||Actin Dependent Regulator Of Chromatin||Subfamily A||Member 4||Mitotic Growth And Transcription Activator||ATP-Dependent Helicase SMARCA4||BRG1||Global Transcription Activator Homologous Sequence Sucrose Nonferme
Purification
Greater than 80.0% as determined by SDS-PAGE.
Components
SMARCA4 protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MGSDPVEILQ EREYRLQARI AHRIQELENL PGSLAGDLRT KATIELKALR LLNFQRQLRQ EVVVCMRRDT ALETALNAKA YKRSKRQSLR EARITEKLEK QQKIEQERKR RQKHQEYLNS ILQHAKDFKE YHRSVTGKIQ KLTKAVATYH ANTEREQKKE NERIEKERMR RLMAEDEEGY RKLIDQKKDK R.

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