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GCA Recombinant Protein

CAT: 0793-32-3866-10Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0793-32-3866-10Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. GCA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-217.a.) and having a molecular mass of 26.1kDa. GCA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Grancalcin is calcium-binding protein which is particularly abundant in human neutrophils. GCA is a member of the penta EF-hand (PEF) subfamily of EF-hand proteins, who also comprises calpain, sorcin, peflin, and ALG-2. GCA undergoes essential conformational changes upon binding of calcium, which subsequently exposes hydrophobic amino acid residues, that direct the protein to hydrophobic surfaces. GCA cooperates with L-plastin, a protein known to have actin bundling activity, which suggests that GCA has a part in the regulation of neutrophils adhesion.
Product Name Alternative
Grancalcin EF-hand calcium binding protein||GCL||Grancalcin penta-EF-hand protein.
Purification
Greater than 95% as determined by SDS-PAGE.
Components
The GCA protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl and 20% glycerol.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTVE HHELRQAIGL MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA CCVKLRALTD FFRKRDHLQQ GSANFIYDDF LQGTMAI

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