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PRKAB2 Recombinant Protein

CAT: 0793-32-3100-20Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0793-32-3100-20Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : E.coli. PRKAB2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 296 amino acids (1-272) and having a molecular mass of 32.8kDa. PRKAB2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. PRKAB2 is a regulatory subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer contains an alpha catalytic subunit and non-catalytic beta and gamma subunits. AMPK is a significant energy-sensing enzyme that supervises cellular energy status. AMPK is activated as a reply to cellular metabolic stresses, therefore phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), vital enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. PRKAB2 is a positive regulator of AMPK activity and highly expressed in skeletal muscle.
Product Name Alternative
5'-AMP-activated protein kinase subunit beta-2||AMPK subunit beta-2.
Purification
Greater than 90% as determined by SDS-PAGE.
Components
The PRKAB2 solution (1mg/mL) contains 20mM Tris-HCl buffer, (pH8.0), 10% glycerol and 2M Urea.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MGSHMGNTTS DRVSGERHGA KAARSEGAGG HAPGKEHKIM VGSTDDPSVF SLPDSKLPGD KEFVSWQQDL EDSVKPTQQA RPTVIRWSEG GKEVFISGSF NNWSTKIPLI KSHNDFVAIL DLPEGEHQYK FFVDGQWVHD PSEPVVTSQL GTINNLIHVK KSDFEVFDAL KLDSMESSET SCRDLSSSPP GPYGQEMYAF RSEERFKSPP ILPPHLLQVI LNKDTNISCD PALLPEPNHV MLNHLYALSI KDSVMVLSAT HRYKKKYVTT LLYKPI.

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