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PPM1G Recombinant Protein

CAT: 0793-32-2713-50Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0793-32-2713-50Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. PPM1G Human Recombinant fused with His-tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 250 amino acids and having a molecular mass of 27 kDa.The PPM1G is purified by proprietary chromatographic techniques. PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.
Product Name Alternative
Protein Phosphatase 1G||PP2CG||PPP2CG||MGC1675||MGC2870||PP2C GAMMA||EC 3.1.3.16||Protein phosphatase 2C isoform gamma||PP2C-gamma||Protein phosphatase magnesium-dependent 1 gamma||Protein phosphatase 1C||PPM1G||PPM1C.
Purification
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Components
The PPM1G solution (1mg/mL) contains 25mM Tris pH-7.5, 1mM DTT, 1mM EDTA, 2mM b-ME and 20% glycerol.
Storage Conditions
PPM1G although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD.