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LDHB Recombinant Protein

CAT: 0793-32-2502-3Size: 3 mgDry Ice: NoHazardous: No
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CAT#:0793-32-2502-3Size:3 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart. Lactate dehydrogenase (LDH) is an enzyme (EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Product Name Alternative
Lactate Dehydrogenase||LDH.
Purification
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Components
The protein (1 mg/mL) was lyophilized with 0.1mg potassium phosphate.
Storage Conditions
Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Applications Notes
It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. The specific activity was found to be 258 U/mg protein.

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