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ALKBH2 Recombinant Protein

CAT: 0793-32-2149-20Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0793-32-2149-20Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. ALKBH2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a.) and having a molecular mass of 31.4kDa.ALKBH2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. ALKBH2 is a member of the ALKB family.ALKBH2 is expressed in the heart, colon, liver, testis, ovary, prostate and the small intestine. ALKBH2 uses iron as a cofactor and acts as a dioxygenase which catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and Alpha-ketoglutarate for enzymatic activity.
Product Name Alternative
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2||Alkylated DNA repair protein alkB homolog 2||Oxy DC1||ALKBH2||ABH2||FLJ99103||MGC90512.
Purification
Greater than 90.0% as determined by SDS-PAGE.
Components
ALKBH2 solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 30% glycerol.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K.

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