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MSCF Recombinant Protein

CAT: 0793-32-1744-10Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0793-32-1744-10Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. Stem Cell Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18309 Dalton. The SCF is purified by proprietary chromatographic techniques. Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117 (c-Kit) . SCF is also known as 'steel factor' or 'c-kit ligand'. SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid) .
Product Name Alternative
Kit ligand Precursor||C-kit ligand||SCF||Mast cell growth factor||MGF||SF||KL-1||Kitl||DKFZp686F2250||Hematopoietic growth factor KL||Steel factor.
Purification
Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Components
Lyophilized from a concentrated (1mg/mL) solution in water containing 0.02% NaHCO3.
Storage Conditions
Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.
Applications Notes
It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cell line is < 10 ng/ml, corresponding to a Specific Activity of 1x105 IU/mg.
Amino Acids
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.

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