MRELM b Recombinant Protein

Source : Escherichia Coli. Mouse RELM-b Recombinant produced in E.Coli is a monomeric, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 8.9kDa. The Mouse RETNLB is purified by proprietary chromatographic techniques. RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin (also called FIZZ3), RELM-alfa (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents.RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant.The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.

Resistin-like beta||RELM beta||Cysteine-rich secreted protein FIZZ2||Colon and small intestine-specific cysteine-rich protein||Cysteine-rich secreted protein A12-alpha-like 1||Colon carcinoma-related gene protein||RELM-b||XCP2||HXCP2.

Greater than 97% as determined by SDS-PAGE.

The protein was lyophilized from a concentrated (1mg/mL) protein solution containing 10mM Acetic Acid with 2:1 mannitol to protein.

Lyophilized RETNLB is stable at -20°C. After reconstitution the protein should be kept at all times at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Reconstitute at 0.1 mg/ml with sterile pyrogen free water.