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IL36B Recombinant Protein

CAT: 0793-32-1529-10Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0793-32-1529-10Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. IL36B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17.7kDa.The IL36B is purified by proprietary chromatographic techniques. Human IL-36b belongs to the IL-1 family that includes IL-1b, IL-1a, IL-1ra, IL-18, IL-36ra (IL1F5), IL-36b (IL1F8), IL-36g (IL1F9), IL-37 (IL1F7) and IL-38 (IL-1F10) . The IL-1 family members display a 12 b-strand, b-trefoil configuration, and are thought to have ascended from a mutual ancestral gene. IL-36 beta is known to be actively secreted. Cells expressing IL-36 beta include resting and activated monocytes and B cells. The receptor for IL-36 beta is a blend of IL-1 Rrp2 and IL-1 RAcP. Recombinant IL-36 beta stimulates processes involving NF-kB and MAPK in an IL-1 Rrp2-dependent manner.
Product Name Alternative
Interleukin 36 beta||interleukin 1 family member 8 (eta) ||Interleukin-1 homolog 2||IL1F8 (Canonical product IL-1F8a) ||IL-1F8 (FIL1-eta) ||Interleukin-1 Superfamily e||IL1H2||MGC126880||MGC126882.
Purification
Greater than 95.0% as determined by SDS-PAGE.
Components
Lyophilized from a 0.2μm filtered concentrated solution in 1xPBS, pH 7.4.
Storage Conditions
Lyophilized IL36B Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36B should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Applications Notes
It is recommended to reconstitute the lyophilized IL36B in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Measured by its binding ability in a functional ELISA to bind recombinant human IL-1 Rrp2 Fc Chimera.
Amino Acids
MNPQREAAPK SYAIRDSRQM VWVLSGNSLI AAPLSRSIKP VTLHLIACRD TEFSDKEKGN MVYLGIKGKD LCLFCAEIQG KPTLQLKEKN IMDLYVEKKA QKPFLFFHNK EGSTSVFQSV SYPGWFIATS TTSGQPIFLT KERGITNNTN FYLDSVE

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