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Anti-Human CD73 APC (Clone : AD2)

CAT: 0793-30-2549-100Size: 100 TestsDry Ice: NoHazardous: No
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CAT#:0793-30-2549-100Size:100 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD73 (ecto-5 ́-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.Specificity : The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5 ́-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.
Product Name Alternative
Ecto-5'nucleotidase, NT5E, E5NT, CALJA, NTE,5'-nucleotidase ecto
Gene Name
NT5E
Gene ID
4907
Reactivity
Human
Target Antigen
CD73
Clonality
Monoclonal
Clone
AD2
Applications
FACS
Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Components
Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage Conditions
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Applications Notes
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Isotype
Mouse IgG1 kappa
Conjugate
APC

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