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Polyclonal Antibody to Beta actin

CAT: 0793-11-13012-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0793-11-13012-100Size:100 µg
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Description
B-actin (ACTB) has traditionally been regarded as an endogenous housekeeping gene and has been widely used as a reference gene/protein in quantifying expression levels in tumors. It supports fundamental cellular processes in healthy and diseased cells including cell adhesion, migration, cytokinesis and maintenance of cell polarity. However, B-actin is closely associated with a variety of cancers and accumulating evidence indicates that B-actin is de-regulated in liver, melanoma, renal, colorectal, gastric, pancreatic, esophageal, lung, breast, prostate, ovarian cancers, leukemia and lymphoma. This protein is generally found to be up-regulated in the majority of tumor cells and tissues.
Product Name Alternative
Actin, cytoplasmic 1, ACTB
Gene Name
ACTB
Gene ID
60
UniProt
P60709
Host
Rabbit
Reactivity
Rat, Mouse, Human
Immunogen
A partial length recombinant Beta actin protein (amino acids 1-200) was used as the immunogen for this antibody.
Target Antigen
Beta actin
Clonality
Polyclonal
Applications
WB
Purification
Protein A Chromatography
Format
Purified
Components
25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Western blot analysis: 4-6 μg/mL:
Isotype
Rabbit IgG

UniProtKB · P60709

Actin, cytoplasmic 1

ACTB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P60709
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACTB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.6.4.-
Processing
—
Secondary accessions
P02570, P70514, P99021, Q11211, Q64316, Q75MN2, Q96B34, Q96HG5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationOxidationUbl conjugation

Ligand

ATP-bindingNucleotide-binding

Cellular component

CytoplasmCytoskeletonNucleus

Disease

DeafnessDisease variantDystoniaIntellectual disability

Molecular function

Hydrolase

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E55A04596ACTB/β-actin/Beta Actin Polyclonal Antibody
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