Rapamycin

• Description:

  Rapamycin (Sirolimus; AY 22989) is a potent and specific mTOR inhibitor with an IC50 of 0.1 nM in HEK293 cells. Rapamycin binds to FKBP12 and specifically acts as an allosteric inhibitor of mTORC1[1]. Rapamycin is an autophagy activator, an immunosuppressant[2].

• Product Name Alternative:

  Sirolimus; AY-22989; NSC 226080

• UNSPSC:

  12352005

• Hazard Statement:

  H302, H315, H319, H335

• Target:

  Antibiotic; Autophagy; Bacterial; Endogenous Metabolite; FKBP; Fungal; mTOR

• Type:

  Natural Products

• Related Pathways:

  Anti-infection; Apoptosis; Autophagy; Cell Cycle/DNA Damage; Immunology/Inflammation; Metabolic Enzyme/Protease; PI3K/Akt/mTOR

• Applications:

  Cancer-Kinase/protease

• Field of Research:

  Cancer; Infection; Neurological Disease; Metabolic Disease; Inflammation/Immunology

• Assay Protocol:

  https://www.medchemexpress.com/Rapamycin.html

• Purity:

  99.94

• Solubility:

  DMSO : 100 mg/mL (ultrasonic)

• Smiles:

  O=C ([C@@]1 (O) [C@@H] (CC[C@@H] (C[C@@H] (/C (C) =C/C=C/C=C/[C@H] (C[C@@H] (C) C ([C@@H] ([C@@H] (/C (C) =C/[C@H]2C) O) OC) =O) C) OC) O1) C) C (N3CCCC[C@H]3C (O[C@@H] (CC2=O) [C@@H] (C[C@H]4C[C@H] ([C@H] (O) CC4) OC) C) =O) =O

• Molecular Formula:

  C51H79NO13

• Molecular Weight:

  914.17

• Precautions:

  H302, H315, H319, H335

• References & Citations:

  [1]Edwards SR, et al. The rapamycin-binding domain of the protein kinase mammalian target of rapamycin is a destabilizing domain. J Biol Chem, 2007, 282 (18), 13395-13401.|[2]Rangaraju S, et al. Rapamycin activates autophagy and improves myelination in explant cultures from neuropathicmice. J Neurosci. 2010 Aug 25;30 (34) :11388-97.|[3]Niu H, et al. Rapamycin potentiates cytotoxicity by RP-56976 possibly through downregulation of Survivin in lung cancer cells. J Exp Clin Cancer Res. 2011 Mar 10;30:28.|[4]Zhang JW, et al. Metformin synergizes with rapamycin to inhibit the growth of pancreatic cancer in vitro and in vivo. Oncol Lett. 2018 Feb;15 (2) :1811-1816.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  -20°C, 3 years; 4°C, 2 years (Powder)

• Scientific Category:

  Natural Products

• Clinical Information:

  Launched

• Isoform:

  FKBP12; Microbial Metabolite; mTORC1

• CAS Number:

  53123-88-9