VEGFA Protein (Native, Human)

• Abbreviation :

  VEGFA

• Synonyms :

  L-VEGF, MVCD1, VEGF, VEGF-A, VPF

• Host :

  HEK 293

• Tag :

  Native

• Endotoxin :

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity :

  > 95 % as determined by SDS-PAGE

• Bioactivity :

  1. Measured by its binding ability in a functional ELISA. Immobilized human VEGF121b (Cat:15692-HNAH) at 10μg/mL (100μL/well) can bind human VEGFR2-Fc (Cat:10012-H02H), the EC50 of human VEGFR2-Fc is 0.06-0.1 μg/mL. 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 1-5ng/mL.

• Function :

  Met1-Asp147

• Molecular Weight :

  The recombinant human VEGF consists of 121 amino acids and has a predicted molecular mass of 14 kDa. The apparent molecular mass of the human VEGF121b is approximately 20 and 16 kDa in SDS-PAGE under reducing conditions due to glycosylation.

• Species :

  Human