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SLIT2 Protein (C-His, Human)

CAT: 1543-P528531Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1543-P528531Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
SLIT2
Synonyms
SLIL3, Slit-2
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Recombinant Human SLIT2 Protein (His Tag) (Cat: 11967-H08H) at 10 μg/mL (100 μL/well) can bind Recombinant Human ROBO1 Protein (ECD, Fc Tag) (Cat: 30073-H02H), the EC50 is 60-180 ng/mL. 2.Loaded Recombinant Human ROBO1 Protein, hFc Tag (Cat. No. 30073-H02H) on proA Biosensor, can bind Recombinant Human SLIT2 Protein, His Tag (Cat. No. 11967-H08H) with an affinity constant of 42.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Leu271-Ala480
Molecular Weight
The recombinant Human SLIT2 consisting of 221 amino acids and has a calculated molecular mass of 24.98 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 23.5 kDa protein in SDS-PAGE under reducing conditions.
Species
Human