IL3 Protein (Native, Mouse)

• Abbreviation :

  IL3

• Synonyms :

  BPA, Csfmu, HCGF, Il-3, Il3, MCGF, PSF

• Host :

  HEK 293

• Tag :

  Native

• Endotoxin :

  < 50 EU per mg protein.

• Purity :

  ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

• Bioactivity :

  1.Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.1-0.5 ng/mL. 2.Loaded Recombinant Mouse IL-3R alpha/CD123 Protein, His Tag (Cat. No. 50810-M08H) on NTA Biosensor, can bind Recombinant Mouse IL-3 Protein (Cat. No. 51066-MNAH) with an affinity constant of 0.382 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Recombinant Mouse IL-3R alpha/CD123 Protein, His Tag (Cat. No. 50810-M08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Mouse IL-3 Protein (Cat. No. 51066-MNAH) with an affinity constant of 2.202 μM as determined in an SPR assay (Biacore T200) (QC tested) .

• Function :

  Met1-Cys166

• Molecular Weight :

  The recombinant mouse IL3 consists of 140 amino acids and predicts a molecular mass of 15.7 kDa.

• Species :

  Mouse