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CD86 Protein (C-His, Human)

CAT: 1543-P520830Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1543-P520830Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
CD86
Synonyms
B7-2, B7.2, B70, CD28LG2, LAB72
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 97 % as determined by SDS-PAGE
Bioactivity
1.Measured by its binding ability in a functional ELISA. Immobilized human CD86 at 20 μg/ml (100 μl/well) can bind human CD28 with a linear range of 32-800 ng/ml. 2.Loaded Recombinant Human B7-1 Protein, His Tag (Cat. No. 10698-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 0.46 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 3.Loaded Recombinant Human CD86 Protein, His Tag (Cat. No. 10699-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 2.72 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Met1-His239
Molecular Weight
The recombinant human B7-2 consists of 231 amino acids and has a predicted molecular mass of 26.6 kDa. In SDS-PAGE, the apparent molecular mass of rhB7-2 is approximately 55-60 kDa due to glycosylation.
Species
Human

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